Trade-offs between biomass growth and inducible biosynthesis of polyhydroxybutyrate in transgenic poplar.
Identifieur interne : 002C70 ( Main/Exploration ); précédent : 002C69; suivant : 002C71Trade-offs between biomass growth and inducible biosynthesis of polyhydroxybutyrate in transgenic poplar.
Auteurs : David A. Dalton [États-Unis] ; Cathleen Ma ; Shreya Shrestha ; Peter Kitin ; Steven H. StraussSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2011.
Descripteurs français
- KwdFr :
- Biomasse (MeSH), Biopolymères (biosynthèse), Chlorophylle (métabolisme), Ecdysone (pharmacologie), Facteurs temps (MeSH), Feuilles de plante (croissance et développement), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Feuilles de plante (ultrastructure), Gènes bactériens (génétique), Génie génétique (MeSH), Hydroxy-butyrates (métabolisme), Mutation (MeSH), Polymères (MeSH), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Populus (ultrastructure), Racines de plante (croissance et développement), Racines de plante (génétique), Racines de plante (métabolisme), Racines de plante (ultrastructure), Régulation de l'expression des gènes végétaux (MeSH), Tiges de plante (croissance et développement), Tiges de plante (génétique), Tiges de plante (métabolisme), Tiges de plante (ultrastructure), Transgènes (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme), Végétaux génétiquement modifiés (ultrastructure).
- MESH :
- biosynthèse : Biopolymères.
- croissance et développement : Feuilles de plante, Populus, Racines de plante, Tiges de plante, Végétaux génétiquement modifiés.
- génétique : Feuilles de plante, Gènes bactériens, Populus, Racines de plante, Tiges de plante, Végétaux génétiquement modifiés.
- métabolisme : Chlorophylle, Feuilles de plante, Hydroxy-butyrates, Populus, Racines de plante, Tiges de plante, Végétaux génétiquement modifiés.
- pharmacologie : Ecdysone.
- ultrastructure : Biomasse, Facteurs temps, Feuilles de plante, Génie génétique, Mutation, Polymères, Populus, Racines de plante, Régulation de l'expression des gènes végétaux, Tiges de plante, Transgènes, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Biomass (MeSH), Biopolymers (biosynthesis), Chlorophyll (metabolism), Ecdysone (pharmacology), Gene Expression Regulation, Plant (MeSH), Genes, Bacterial (genetics), Genetic Engineering (MeSH), Hydroxybutyrates (metabolism), Mutation (MeSH), Plant Leaves (genetics), Plant Leaves (growth & development), Plant Leaves (metabolism), Plant Leaves (ultrastructure), Plant Roots (genetics), Plant Roots (growth & development), Plant Roots (metabolism), Plant Roots (ultrastructure), Plant Stems (genetics), Plant Stems (growth & development), Plant Stems (metabolism), Plant Stems (ultrastructure), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Plants, Genetically Modified (ultrastructure), Polymers (MeSH), Populus (genetics), Populus (growth & development), Populus (metabolism), Populus (ultrastructure), Time Factors (MeSH), Transgenes (MeSH).
- MESH :
- chemical , biosynthesis : Biopolymers.
- chemical , metabolism : Chlorophyll, Hydroxybutyrates.
- chemical , pharmacology : Ecdysone.
- genetics : Genes, Bacterial, Plant Leaves, Plant Roots, Plant Stems, Plants, Genetically Modified, Populus.
- growth & development : Plant Leaves, Plant Roots, Plant Stems, Plants, Genetically Modified, Populus.
- metabolism : Plant Leaves, Plant Roots, Plant Stems, Plants, Genetically Modified, Populus.
- ultrastructure : Plant Leaves, Plant Roots, Plant Stems, Plants, Genetically Modified, Populus.
- Biomass, Gene Expression Regulation, Plant, Genetic Engineering, Mutation, Polymers, Time Factors, Transgenes.
Abstract
Polyhydroxybutyrate (PHB) is a bioplastic that can be produced in transgenic plants by the coexpression of three bacterial genes for its biosynthesis. PHB yields from plants have been constrained by the negative impacts on plant health that result from diversion of resources into PHB production; thus, we employed an ecdysone analogue-based system for induced gene expression. We characterized 49 insertion events in hybrid transgenic poplar (Populus tremula x alba) that were produced using Agrobacterium transformation and studied two high-producing events in detail. Regenerated plants contained up to 1-2% PHB (dry weight) in leaves after 6-8 weeks of induction. Strong induction was observed with 1-10 mm Intrepid and limited direct toxicity observed. Confocal fluorescence microscopy was used to visualize PHB granules in chloroplasts after chemical treatment to reduce autofluorescence. A greenhouse study indicated that there were no negative consequences of PHB production on growth unless the PHB content exceeded 1% of leaf weight; at PHB levels above 1%, growth (height, diameter and total mass) decreased by 10%-34%.
DOI: 10.1111/j.1467-7652.2010.00585.x
PubMed: 21265995
Affiliations:
Links toward previous steps (curation, corpus...)
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(genetics)</term><term>Plant Stems (growth & development)</term><term>Plant Stems (metabolism)</term><term>Plant Stems (ultrastructure)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plants, Genetically Modified (ultrastructure)</term><term>Polymers (MeSH)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>Populus (ultrastructure)</term><term>Time Factors (MeSH)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biomasse (MeSH)</term><term>Biopolymères (biosynthèse)</term><term>Chlorophylle (métabolisme)</term><term>Ecdysone (pharmacologie)</term><term>Facteurs temps (MeSH)</term><term>Feuilles de plante (croissance et développement)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Feuilles de plante (ultrastructure)</term><term>Gènes bactériens (génétique)</term><term>Génie génétique (MeSH)</term><term>Hydroxy-butyrates (métabolisme)</term><term>Mutation (MeSH)</term><term>Polymères (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Populus (ultrastructure)</term><term>Racines de plante (croissance et développement)</term><term>Racines de plante (génétique)</term><term>Racines de plante (métabolisme)</term><term>Racines de plante (ultrastructure)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Tiges de plante (croissance et développement)</term><term>Tiges de plante (génétique)</term><term>Tiges de plante (métabolisme)</term><term>Tiges de plante (ultrastructure)</term><term>Transgènes (MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés 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modifiés</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Ecdysone</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Plant Leaves</term><term>Plant Roots</term><term>Plant Stems</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Gene Expression Regulation, Plant</term><term>Genetic Engineering</term><term>Mutation</term><term>Polymers</term><term>Time Factors</term><term>Transgenes</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Biomasse</term><term>Facteurs temps</term><term>Feuilles de plante</term><term>Génie génétique</term><term>Mutation</term><term>Polymères</term><term>Populus</term><term>Racines de plante</term><term>Régulation de l'expression des gènes végétaux</term><term>Tiges de plante</term><term>Transgènes</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polyhydroxybutyrate (PHB) is a bioplastic that can be produced in transgenic plants by the coexpression of three bacterial genes for its biosynthesis. PHB yields from plants have been constrained by the negative impacts on plant health that result from diversion of resources into PHB production; thus, we employed an ecdysone analogue-based system for induced gene expression. We characterized 49 insertion events in hybrid transgenic poplar (Populus tremula x alba) that were produced using Agrobacterium transformation and studied two high-producing events in detail. Regenerated plants contained up to 1-2% PHB (dry weight) in leaves after 6-8 weeks of induction. Strong induction was observed with 1-10 mm Intrepid and limited direct toxicity observed. Confocal fluorescence microscopy was used to visualize PHB granules in chloroplasts after chemical treatment to reduce autofluorescence. A greenhouse study indicated that there were no negative consequences of PHB production on growth unless the PHB content exceeded 1% of leaf weight; at PHB levels above 1%, growth (height, diameter and total mass) decreased by 10%-34%.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21265995</PMID><DateCompleted><Year>2011</Year><Month>12</Month><Day>01</Day></DateCompleted><DateRevised><Year>2011</Year><Month>08</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>7</Issue><PubDate><Year>2011</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Trade-offs between biomass growth and inducible biosynthesis of polyhydroxybutyrate in transgenic poplar.</ArticleTitle><Pagination><MedlinePgn>759-67</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1467-7652.2010.00585.x</ELocationID><Abstract><AbstractText>Polyhydroxybutyrate (PHB) is a bioplastic that can be produced in transgenic plants by the coexpression of three bacterial genes for its biosynthesis. PHB yields from plants have been constrained by the negative impacts on plant health that result from diversion of resources into PHB production; thus, we employed an ecdysone analogue-based system for induced gene expression. We characterized 49 insertion events in hybrid transgenic poplar (Populus tremula x alba) that were produced using Agrobacterium transformation and studied two high-producing events in detail. Regenerated plants contained up to 1-2% PHB (dry weight) in leaves after 6-8 weeks of induction. Strong induction was observed with 1-10 mm Intrepid and limited direct toxicity observed. Confocal fluorescence microscopy was used to visualize PHB granules in chloroplasts after chemical treatment to reduce autofluorescence. A greenhouse study indicated that there were no negative consequences of PHB production on growth unless the PHB content exceeded 1% of leaf weight; at PHB levels above 1%, growth (height, diameter and total mass) decreased by 10%-34%.</AbstractText><CopyrightInformation>© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dalton</LastName><ForeName>David A</ForeName><Initials>DA</Initials><AffiliationInfo><Affiliation>Biology Department, Reed College, Portland, OR, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Cathleen</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Shrestha</LastName><ForeName>Shreya</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Kitin</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>01</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001704">Biopolymers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006885">Hydroxybutyrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011108">Polymers</NameOfSubstance></Chemical><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>3604-87-3</RegistryNumber><NameOfSubstance UI="D004440">Ecdysone</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001704" MajorTopicYN="N">Biopolymers</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004440" MajorTopicYN="N">Ecdysone</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005798" MajorTopicYN="N">Genes, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006885" MajorTopicYN="N">Hydroxybutyrates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011108" MajorTopicYN="N">Polymers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>12</Month><Day>13</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21265995</ArticleId><ArticleId IdType="doi">10.1111/j.1467-7652.2010.00585.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Kitin, Peter" sort="Kitin, Peter" uniqKey="Kitin P" first="Peter" last="Kitin">Peter Kitin</name><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name><name sortKey="Shrestha, Shreya" sort="Shrestha, Shreya" uniqKey="Shrestha S" first="Shreya" last="Shrestha">Shreya Shrestha</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Dalton, David A" sort="Dalton, David A" uniqKey="Dalton D" first="David A" last="Dalton">David A. Dalton</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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